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How to remove a file lock manually in MS Server 2008

Open AdministrativeTools -> Share and Storage Management.

 From the Actions pane, click Manage Open Files. 

 Here you are able to see all locked files and folders on this server.  To clear a lock, select the relavent file and click “Close Selected”.

Note that this should only be done if the user definitely is not using the the file, otherwise data loss may occur.